Non Linear Fit of RAW amplitudes Non Linear Fit of Normalized
P[sytWT] P[sytA-ME] P[sytA-ME,FE] P[sytWT] P[sytA-ME] P[sytA-ME,FE]
[Agonist] vs. response -- Variable slope (four parameters) [Agonist] vs. normalized response -- Variable slope
Best-fit values Best-fit values
Bottom 1.665 1.257 0.3403 HillSlope 1.729 1.665 1.652
Hillslope 1.758 1.755 1.803 EC50 0.6286 0.9271 0.9843
Top 37.08 32.19 7.928 logEC50 -0.2016 -0.0329 -0.00689
EC50 0.6663 1.007 1.05 Std. Error
logEC50 -0.1763 0.003072 0.02132 HillSlope 0.1413 0.1046 0.1753
Span 35.42 30.94 7.587 EC50 0.03268 0.03583 0.06383
Std. Error 95% CI (profile likelihood)
Bottom 0.9541 0.7287 0.4123 HillSlope 1.389 to 2.171 1.412 to 1.975 1.253 to 2.216
Hillslope 0.1803 0.1908 0.4662 EC50 0.5468 to 0.7171 0.8379 to 1.023 0.8288 to 1.160
Top 1.068 1.203 0.7009 logEC50 -0.2622 to -0.1444 -0.07683 to 0.009701 -0.08153 to 0.06436
EC50 0.04318 0.06619 0.159 Goodness of Fit
Span 1.624 1.61 0.9295 Degrees of Freedom 5 5 5
95% CI (profile likelihood) R squared 0.9928 0.9954 0.9869
Bottom -1.775 to 4.558 -1.304 to 3.408 -2.497 to 1.470 Sum of Squares 59.24 35.46 100.6
Hillslope 1.214 to 2.483 1.195 to 2.506 0.3795 to 5.544 Sy.x 3.442 2.663 4.486
Top 34.09 to 41.64 28.94 to 37.89 6.286 to 63.18 Constraints
EC50 0.5431 to 0.8277 0.8225 to 1.313 0.6581 to 377.7 EC50 EC50 > 0 EC50 > 0 EC50 > 0
logEC50 -0.2651 to -0.08212 -0.08488 to 0.1183 -0.1817 to 2.577
Goodness of Fit Number of points
Degrees of Freedom 3 3 3 # of X values 7 7 7
R squared 0.9979 0.998 0.9889 # Y values analyzed 7 7 7
Sum of Squares 2.26 1.589 0.5373
Sy.x 0.868 0.7279 0.4232
Constraints
EC50 EC50 > 0 EC50 > 0 EC50 > 0
Number of points
# of X values 7 7 7
# Y values analyzed 7 7 7